Journal: bioRxiv
Article Title: Divergent use of metabolic fluxes in breast cancer metastasis
doi: 10.1101/2020.08.03.234468
Figure Lengend Snippet: A) RNA expression of LDHA and LDHB in BrM2 vs parental cells. LDHA: 1.5-fold, p=0.3, LDHB: 1.1-fold, p=0.3. Data are represented as mean ± SD. B) RNA expression of LDHA and LDHB in LM2 vs parental cells. LDHA: .9-fold, p=0.7. LDHB: 1.4-fold, p=0.02. Data are represented as mean ± SD. C) Boxplot of ratio of LDH genes (sum of LDHA and LDHB) to PDH genes (sum of PDHA1 and PDHA2) in cell lines. Fold change relative to parental: BrM2: 1.2-fold, p=0.2, LM2: 1.5-fold, p=0.007. Fold change LM2/BrM2: 1.3-fold, p=0.03. D) Boxplot of ratio of LDH genes to PDH genes in patient tumors from The Metastatic Breast Cancer Project. The “Lung” classification includes all tumors that had lung metastases, “Brain” classification includes all tumors that had brain/CNS metastases, and “Other” classification contains tumors that had metastases other than to the lung or brain/CNS. Fold change lung/other: 1.6-fold, p=0.02. See also Fig S5.
Article Snippet: The ratio of gene expression of (LDHA+LDHB)/(PDHA1+PDHA2) was calculated for each patient; boxplot and statistical analysis of fold change between groups (two-sample t -test) was done in MATLAB.
Techniques: RNA Expression